Polycystic Ovary Syndrome

Research statement

PCOS is one of the most common health disorders affecting women. The PCOS ovary makes too much testosterone and supports increased hair growth on the face and body. The enlarged ovary also grows too many egg-containing follicles, thus providing the enigmatic appearance of the polycystic ovary. PCOS follicles usually fail to mature and frequently fail to release an egg at ovulation, hence the lack of menstrual cycles and infertility associated with the disorder. In addition, PCOS overly contributes to obesity, new cases of type 2 diabetes among young women, gestational diabetes, sleep apnea and metabolic syndrome. All of this increase a woman's lifetime risk of cardiovascular disease.

Research description

Polycystic ovary syndrome, or PCOS, is a common and complex condition affecting 4 to 10 percent of all women of reproductive age. It was once defined as a condition in which the ovaries develop tiny cysts, often becoming enlarged. All women with PCOS have other features in common, however: all are premenopausal, and all have high levels of the virilizing hormone androgen, which interfere with the normal release of eggs from the ovaries (ovulation). As a result, they often have symptoms associated with high levels of male hormones, including masculinization, irregular menstrual periods, and fertility problems. In addition, these symptoms cannot be explained by or attributed to any other conditions. We have used the American Journal of metabolism and Pysiological Endocrinology, publised in the year 2005, for studying the effects, risks, and symptoms associated with Polycystic ovary syndrome.

Methodology

The study included seven hundred and fifty two women, out of which on hundred and forty six were diagnosed with PCOS and six hundred and six, were foreign women were no diabetic controls from a previous sovereign study conducted of the whole population.

A sample of the genomic DNA was taken from the groups, and then it was isolated from their blood, afterwards the genomic DNA was extracted from leukocytes controls of the blood of the groups. Then it was distributed among 8 variants of CAPN10 with different names assigned to them for identification.

The researchers, in the next step extracted 8 SNP's, with very small genetic disparities, which may arise in the sequence of the DNA, as they are associated with the PCOS. Genotyping using DNA analysis for the determination of predisposition among individuals for a certain diseases was performed.

For estimating the genetic relationship of all the eight SNPs along with the PCOS, a difference was measured between the control and the genotype groups, and the impact of the differences in body mass index, and age was determined for both the groups. For clarifying the alleged association between PCOS and CAPN10, researchers performed Meta analysis, with the help of the collected data, along with all the published date, which showed a strong genetic association between PCOS and CAPN10.

Limitations

The researchers had some difficulty in recruitment of the women leading to a limitation of the study which was originally planned, and it did not allowed to conclude in the whole ...