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The development of a single cell into a blastocyst

The development of the single cell in blastocyst takes place when the embryonic stem cell driven from blastocyst establishes in vitro and attach them with the blastocyst cell with the assistance of the tumors. These cells are maintained in a different pluripotent condition and culture themselves in an embryonic layer of feeder. In the absence of this layer these cells differentiate themselves spontaneously. Several researches have been conducted with the successful results which defines the process and development of these cells into a blastocyst. The development process starts with the division of the zygote cell. The division process is very rapid and the cell starts coupling from one to two, two to four and four to eight and in the end it results in the bunch of cells. During this process of splitting up the results in the mass numbers of cells called the pre-embryo, the pre-embryo cells then starting moving towards uterus. (SHI, W 1984 pp.143-152) After the process of four to five days the morula is shaped into the Blastocyst. After the complete movement and formation the cells shape themselves in hollow ball known as blastocyst.

The structure of the blastocyst (trophoblast and inner cell mass)

The structure of the blastocyst (trophoblast and inner cell mass) the blastocyst is spherical (fig. 1) with an inner cell mass which extends into the cavity of the sphere as a disc of cells, usually with a slightly convex lower margin The blastocyst examined range in diameter from 0-19 to 0-5 mm. A typical blastocyst is 0*35-0 40 mm. in diameter and is very slightly elongated in the direction of the axis through the inner cell mass and an embryonic trophoblast. The inner cell mass of such a blastocyst is just over 0-1 mm. in width and underlies approximately 450 of the total sphere formed by the blastocyst. A readily distinguishable layer of typical trophoblast cells overlies the inner cell mass. The cells of the trophoblast are simple squamous in normal blastocyst. (SWEENEY, L. J., 1984 pp.797-800) Surface views show that these cells are hexagonal in outline. Considerable variation in the shape of trophoblast cells is observed in different blastocyst. In cross-sections of the trophoblast, the cells are generally slightly concave on their inner or basal surface and convex over the nucleus on the outer or free surface.

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