Molecular Biology



Molecular Biology

Clinical Background Of MRSA

Methicillin-resistant Staphylococcus Aureus (MRSA) is a bacterium that causes infections in different parts of the body. It is harder to treat than most strains of Staphylococcus Aureus - or staph - because it is resistant to some commonly used antibiotics. The symptoms of MRSA rely on where one is infected. On occasions, it causes soft infections on the skin, resulting in pimples or boilsHowever it can also be the basis more grave skin infections or infect surgical wounds, blood, lungs and urinary tract. (Reischl, Linde, Metz, Leppmeier, Lehn, 2000)

Staph, can usually be with antibiotics. But for decades, some strains of staph - like MRSA - have become resistant to antibiotics that once destroyed it. MRSA was initially found in 1961. Now unwilling to methicillin, amoxicillin, penicillin, oxacillin, and many other antibiotics. Whereas some antibiotics continue to work, MRSA is continually adapting. Researchers developing new antibiotics have a hard time keeping pace.

The genetic code

DNA is an objective method for genotyping the genetic code (ATCG) is very easy to carry and store and analyzed in a relational database. Recent advances in DNA sequencing technology, including fast, affordable, high-capacity system that made possible the sequencing considered a viable method of entry. (Chambers, 1997)

Sequencing the DNA of the same goals of disparate isolates and cataloging mutation model is an approach called comparative sequencing. Two different strategies were used to provide genotyping data: multilocus sequence input (MLST), which compares the sequence variations in multiple genes of management goals and one locus sequence typing, which compares sequence variation from one end. Molecular testing

A molecular test for detection of MRSA based on the automated DNA extraction and real-time PCR was established. The new test is based on the automated extraction of DNA with the Magna Pure LC instrument (Roche ...