In Vitro Genetic Analysis: Selection and Amplification of Rare Functional Nucleic Acids

In Vitro Genetic Analysis: Selection and Amplification of Rare Functional Nucleic Acids

Introduction

In this paper, the problems are related to the methods for the amplification and in vitro selection of rare functional nucleic acid molecules. The main question is the implementation of effective strategies in pool selection, application and construction (Eadie, McBride, Efcavitch, Hoff, and Cathcart, 1987). Further procedures and techniques must be discussed. Functional ribozymes are selected from the pools of largely non-functional molecules and they have the ability to catalyze a ligation reaction within the first system (Eadie, McBride, ...