FLUORESCENCE MICROSCOPY

Fluorescence Microscopy

Fluorescence Microscopy

Fluorescence Microscopy

In the field of biology, the technique of fluorescence microscopy is widely used to visualize the components of cell such as the cytoskeleton, certain protein and other molecules. By employing the high contrast, there is great chance to visualize the objects having far smaller size in comparison of the resolution range of frequent using light microscopes. Fluorescence microscopy is one of the kinds of photoluminescence mechanism, a quantum mechanical process (Spring, 2003, pp. 548). The process becomes visible when the photon emits during the relaxation phase of molecule, in which electron moves towards ground state from the ...