Chromatography

Chromatography

What it is?

Chromatography is a wide range of physical processes which are employed to analyze or separate compound mixtures. There are several forms of chromatography; however, each form operates on the same basic principle. The components that need to be divided are scattered in two stages; a stationary stage, i.e., a solid or a liquid held up on a solid; and a mobile stage, i.e., a gas or a liquid.

How does it work?

Chromatography is a communal term for a range of laboratory methods for the severance of compound mixtures. It entails dispatching a mixture through a stationary stage that includes an analyst which separates it from different molecules present in the mixture and causes it to be secluded. The answer to the question that how does chromatography work is: The mobile stage penetrates through the stationary stage and holds the mixture components along with it. In the stationary stage, distinct components of the mixture are absorbed at distinct points, while they discontinue moving with the mobile stage. In this way the outcomes of a chromatography are obtained from the instant at which distinct components of the mixture discontinue moving and get separated from the rest of the components (Poole, & Schuette, 2003).

Why is it used in laboratories?

Chromatography is used in different laboratories for different purposes. It is sued for separating the constituent of dyes and inks; it is also often used to separate the colored tinctures in plants and also to ascertain the chemical structure of various materials. Crime laboratories often make use of chromatography for identifying the evidences for solving a criminal case, for example, identifying ink, blood, etc, that are found at the scene of crime. Chromatograms of such evidences are matched with the chromatograms of recognized substances so as to identify them. Chromatography is also used in laboratories by the chemists for tracking the movement of a chemical reaction. They are conveniently able to figure out, by examining the sample marks on the chromatography plate, when the products begin to develop and when the reactants are utilized. Chromatography is also used by biologists and chemists in the laboratories to figure out the compounds that a sample contains, for example plants (Still, et.al, 2001).

Real time cases involving chromatography

Finding solution to mysterious criminal cases has always been mesmerizing to human beings. We like detective novels and crime based dramas as we are curious to figure out who the culprit ...