Capillary Electrophoresis (CE)

Capillary Electrophoresis (CE)

Capillary electrophoresis is an analytical technique for separation based on differences between the mobilities of charged species in an electrolyte solution which may be aqueous or organic, within a small capillary under the influence of an electric field. Among the main advantages of capillary electrophoresis can be cited: the high efficiency, versatility, demand for small sample volumes, the reduced analysis time, minimal consumption of solvents, the low cost of consumables, the ability to interface with various detection systems and the simplicity of the basic instrumentation. Thus, due to the efficiency, selectivity and versatility, this project proposes to demonstrate the application of this technique in different areas. In this sense, there is a proposal to enantioselective analysis of fluvastatin and its major metabolite in biological material in plasma, since there is still no method described in the literature for the analysis of this drug by capillary electrophoresis. Finally, it is proposed the development and validation of a method for analysis of imatinib mesylate in plasma.

Separation Techniques

The method of selection includes steps for obtaining, preparation and possible fractionation of the sample, the application of appropriate analytical technique and treatment of the data obtained. The analytical techniques employed may be grouped into two main groups: techniques of separation and spectroscopic techniques. Spectroscopic techniques provide for each compound tested, one information complex related to specific structural features, second separation techniques are used to resolve components of a mixture and the obtained signal can be used for quantitative or qualitative analytical purposes. It is in the separation techniques in which it is due to its wide use in the field of Biotechnology, Biology and Molecular Biochemistry and other branches of research (Skoog; Holler; Nieman, 1998).

Analytical separations currently are mostly by chromatography and electrophoresis. The chromatography comprises a set of important and diverse methods which allow scientists to separate closely related components in mixtures complex, which in many cases is impossible by other means. Molecules can be separated according to their charges, sizes and molecular weights along with the polarity of their links, redox potential, etc.

Capillary electrophoresis (CE)

Capillary electrophoresis (CE) is an analytical technique which allows separation and quantization of wide range of analyses (ions, peptides, proteins, carbohydrates, steroids, nucleic acids, vitamins, drugs, and cells, etc.) From different areas of biotechnology, industry pharmaceutical, clinical, food and environmental among others. Part of the versatility and effectiveness of this technique because it combines elements of other analytical techniques> these may include using sensors sensitivity and high performance liquid chromatography. Electrophoresis allows the separation of charged molecules: proteins, peptides, amino acids, nucleic acids and nucleotides. It allows under certain conditions (use of micelles of ionic detergents) to separate non-ionic molecules, such as steroid hormones, for example, detection sensitivity.

Capillary Electrophoresis is a technique with elements from the conventional (gel) electrophoresis, the isotacho gel electrophoresis, and chromatography, such as HPLC, with linked to various detectors. These characteristics know the basis for a good future of the capillary electrophoresis (Brian M. Tissue, ...