ANNOTATION OF THE GENOME OF BOMBYX MORI

Annotation of the Genome of Bombyx Mori (Silkworm)

Annotation of the Genome of Bombyx Mori (Silkworm)

Discussion

One of approaches that is commonly used in studies of genetic diversity within and among populations or groups of individuals, and is applied with all kinds of markers and organisms, is based on assessments of individual genotypes within and between populations. In such cases a genetic similarity (or dissimilarity) matrix assembled from all promise pairwise blends of individuals is used to characterize population structure based on relative affinities of each individual to all other individuals tested. This approach needs apt procedures for assessing similarity between individuals, and it is especially helpful in the case of likely linkages between distinct loci. The choice of an befitting coefficient of similarity is a very significant and resolute issue to assess clustering, true genetic similarity between individuals, investigating diversity within populations and studying relationship between populations, because distinct similarity coefficients may yield conflicting results (Mita K, 2003).

Silkworm, Bombyx mori L., domesticated for silk production is an agriculturally significant insect and comprises a large number of geographical races and inbred lines that show considerable variety in their qualitative and quantitative traits. Currently, it is the foremost economic resource for almost 30 million families in nations for example China, India, Vietnam, and Thailand . With the establishment of steady transformation, the silkworm has shown the potential to make pharmaceutically significant proteins in high yield unfastening up new submissions for sericulture in health, agricultural, and industrial fields. Annotation of the silkworm genome started a few years before because of its significance for breeding and genetic studies, for isolating precious genes and promoters and for relative genomics. Mita et al. (2003) first started intensive sequencing of the silkworm genome utilising conveyed sequence tags (ESTs). Recently, this group and a second group described the results of whole-genome shotgun sequencing and supplied public access to the gathered silkworm genome data Genetic linkage mapping has verified to be a powerful tool in genetic studies of numerous organisms. A entire linkage map is essential to efficiently carry out molecular-based investigates for example molecular marker assisted selection, QTL mapping of agronomically significant traits, proposition of heterosis and comprehensive investigations of genomic evolution between lineages. Genetic linkage mapping of the silkworm as a significant insect utilising molecular markers is absolutely crucial for genetic studies and for breeding purposes (Goldsmith, 2005).

Molecular markers are commonly used to characterize genetic diversity within or between populations or groups of individuals because they normally detect high levels of polymorphism. Furthermore, RAPDs and AFLPs are effective in permitting multiple loci to be analyzed for each individual in a lone gel run. In investigating banding patterns of molecular markers, the data normally are coded as -vectors, 1 indicating the occurrence and 0 indicating the nonattendance of a band at a exact place in the gel. With diploid organisms and codominant markers, for example allozymes, RFLPs or SSRs, the banding patterns may be converted to homozygous or heterozygous genotypes at each ...