RECOMBINANT PROTEIN

Production of Recombinant Protein

Production of Recombinant Protein

Introduction

The emergence of plants as a suitable source to create recombinant pharmaceutical proteins has taken on a new market from the past decade (Taylor 2000, p. 1). Presently, their production is done at a lower cost while the quality has been enhanced with safer use and the mammalian proteins used actively are documented (Hellwig, Drossard, Twyman and Fischer, 2004).

Lab Techniques

In order to form a recombinant DNA, a cloning vector is required with a molecule of the DNA that will help in the replication within the living cell (Taylor 2000, p. 1). These vectors are a derivate coming from either plasmids or viruses. These contain those important elements which are necessary for the DNA to replicate, such as the genetic signals for replication and elements of convenience necessary for inseting foreign DNA (Hellwig, Drossard, Twyman and Fischer, 2004).

The Polymerase Chain Reaction directs the replication of any DNA successions which are chosen by the lab experimentalist. The main difference between the two methods is that in molecular cloning is done within the living cell while Polymerase Chain Reaction the DNA is replicated in the test tube when there are no living cells (Hellwig, Drossard, Twyman and Fischer, 2004).

The cloning process of any DNA fragment will be done in seven steps:

Selecting the cloning vector and the host organism

Preparing the DNA vector

Preparing that DNA which is to be cloned

To create the recombinant DNA

To introduce the recombinant DNA into the host organism

To select that organism that contains recombinant DNA

To screen the clones where the DNA has been inserted and the biological properties of the two (Taylor 2000, p. 1).

Commercial Application

The process of recombinant DNA is use in the pharmaceutical industry in order to create medicines from the plants that could be modified to create cures for different types ...