Rapid Molecular Detection of Tuberculosis and Rifampin Resistance



Table of Content

1.Introduction:3

2.1Research Question and Importance:3

2.2Rationale for the Study:4

2.3General Characteristics of the genus Mycobacterium5

2.Methods:7

2.1N-acetly-Lcysteine and Sodium hydroxide before Microscopy and MTB/RIF:8

2.2Microscopy and MTB/RIF:8

2.3Statistical Analysis:9

3Results:9

4Discussion and Conclusions:10

5References13

6Appendix16

Rapid Molecular Detection of Tuberculosis and Rifampin Resistance

Introduction:

Research Question and Importance:

The main objective of this study is examining the rapid molecular detection and resistance of Rifampin. The paper further studies the resistance of tuberculosis to drugs, and particularly MTB (Mycobacterium tuberculosis), which is a serious problem affecting the health of people. It further examines the testing of the use of tuberculosis by the use of MTB/RIF. Resistance of MTB and RIF is disease is highly lethal, especially in people infected with HIV (Drobniewski & Caws, 2001, pp 5-9). Information on patterns of resistance among strains of MTB isolated from patients is an important element of over-visory on the study of tuberculosis. Drug resistant tuberculosis are helpful in detecting and monitoring the spread of tuberculosis (Aziz et al. 2006, pp 2142-2154).

It should be noted that the detection of patients with tuberculosis Leko- refractory requires close cooperation with the medical laboratory. The ability to quickly and reliably diagnose tuberculosis is a complex task. Drug resistance testing performed without the preservation of quality control laboratory may have mistakenly ne results. Sequencing the genome of Mycobacterium tuberculosis enabled a systematic investigation process and progress in understanding the biology of the bacillus, although the origin of its virulence and pathogenicity still remains somewhat obscure.

Genetic engineering has not yet managed to design more effective or appropriate weapons than those already known to control tuberculosis. Neither the varieties of the bacillus created by recombination or mutation, or their antigens or DNA immunogens were significantly better. At the moment, it is not productive search for new vulnerable targets of the organism or active antibiotics, little progress is being made in the way of improving the formulation or route of administration of conventional drugs (Callister et al. 2002, pp 152-156). However, what has developed ingenious and powerful tools to optimize epidemiological tracing and diagnosis of the disease.

The discovery of molecular markers of strains of TB funded research the spread of the organism, and led to the discovery that some of their lineages expand and prevail in certain niches. Molecular methods were accurate to characterize immediately bacilli detected by smear or culture and gave greater range and accuracy in rapid, decisive in the case of patients with immune nosuprimidos MDR-TB. To be logical and true meaning, the results of molecular studies must be supported by the microbiology, clinical pathology and epidemiology traditional.

Rationale for the Study:

Although tuberculosis is one of the deadliest threats to public health today, still lack of effective methods of diagnosis, exacerbating the global problem, since patients with untreated tuberculosis remain a source of infection to other members of the community. Untreated TB also causes considerable morbidity and mortality on particularly among people infected with HIV (Drobniewski et al. 2002, pp 810-816). The most widely used method for TB in most countries where disease is endemic is ...