IMMUNOHISTOCHEMICAL

Immunohistochemical Method & Epstein Barr Virus in Tonsil



Immunohistochemical Method & Epstein Barr Virus in Tonsil

Introduction

As immunohistochemistry refers to the examination of tissues with immunochemical methods, such as staining of cells or tissue structures with on antibody coupled dyes. A distinction is made direct staining, the primary antibody with a marker is staining coupled and indirect, in which a secondary antibody coupled to the marker against fragment is used the primary. However, where said marker is a fluorescent dye, one speaks of immunofluorescence. The principle of the detection reaction occurs through an affinity of antibodies to a very special fabric to pass. A specific property of the tissue (epitope) then triggers from one antibody antigen reaction. In most cases go epitope and the antibody thereby a strong bonding. To the antibody, prior to the addition to the preparation to be examined by means of coupling a specific detection system (detection system) is appended, which is the presence and activity of the antibody is to display. Thus, the detection system, the antibody acts as a detection system of this tissue specific property the epitope. This allows specific tissues (for example) can be distinguished from malignant tissue surrounding gracious tissue (Joseph, et.al, 2000, pp. 9964-9971). The ultimate goal is to achieve a sufficiently strong complex of the epitope, while not part of the epitope associated with tissue sections should remain as unstained. The antibody directed against the epitope.

Discussion

Indirect Method is also called Indirect Immunofluorescence (IIF). Two-step method first a specific primary antibody rose to the preparation to be examined (cells, tissue samples), then application of an antibody that is directed against the previously applied primary antibody. It is a secondary antibody to which an enzyme is coupled to triggering an enzyme-substrate reaction with the formation of a visible dye, which now as an indicator for the epitope is considered to method is useful when a large amount of the epitope to be displayed. Another possible application is the detection of anti-neutrophil cytoplasmic antibodies (ANCA). Whereas, Direct Method refers to get together delivering the protein to be examined, with a specific antibody this primary antibody is directly linked to one enzyme or Flour phosphor (such as Red Texas, fluoresce in or rhodamine conjugated (Chaganti, et.al, 2009, pp. 6372-6381). The antibody-enzyme binds complex to the protein in the next step, which is enzyme a very specific substrate was added, thus causing a inking enzyme-substrate reaction is the formation of the dye is only where the immunohistochemical reaction has taken place this method is very suitable for the display of a plurality of different proteins in a preparation. Other methods APAAP method: using an alkaline phosphatase anti-alkaline phosphatase complex as indicator Labelled (strept) avid in-biotin method (LSAB): Based on the affinity of streptavidin (Streptomyces avidinii ) and avidin (egg white) for biotin. Currently, there is a tendency to an increase in the number of chronic bacterial and viral diseases that are characterised by recurrent and continuous low effectiveness of antibiotic and symptomatic ...