IMMUNOCYTOCHEMISTRY

Immunocytochemical Staining and Haematology

Immunocytochemical Staining and Haematology

Introduction

ICC (Immunocytochemistry) is a technique commonly used in laboratories using antibodies, which targets particular proteins or peptides antigens, by particular epitopes, in the cell. By using several methods, these antibodies are detectable. Immunocytochemistry enables researchers to evaluate whether the cells in certain samples convey the antigen, or not. The Immunocytochemical also enables researchers to identify which sub-cellular sections are conveying the antigen, in cases where an immunopositive signal is establish (Spielman & Brand 1995, pp.70).

Discussion

Jok 1 Cells

Human gastric epithelial cells were immortalized with SV40 (simian virus 40) and secluded from samples of human gastric lining to produce the steady human gastric epithelial “JOK-l” cell line. These cells express conventional epithelial markers such as epithelial specific antigen, mucin, ZO-1, ZO-2, beta-catenin, E-cadherins, occluding, cytokeratin-18, and vimentin, as well as, simian virus 40 large T-antigen. In reaction to acidic medium, these cells quickly externalized E-cadherin, and displayed epithelial-like obstacle properties that are also synchronized by media pH.

In comparison, numerous epithelial markers are expressed by the kidney epithelial cell line “MDCK” (epithelial specific antigen, ZO-1, ZO-2, beta-catenin, E and N-cadherin, occluding, cytokeratin-18, vimentin), but does not convey big T-antigen, or mucin. Nevertheless, E-adherin is internalized rapidly by MDCK from the cell facade, and raises the solute instability in an acidic medium. JOK-1 cell line is a probable helpful cell line, according to data, for making models of gastric epithelial disease, development, and function (Spielman & Brand 1995, pp.70).

HCL (Hairy cell leukaemia) is a persistent B-lymphocyte leukaemia. HCL-derived cell lines are vital tool for researches on this illness, due to the proliferative inaction of the malignant cells. The karyotypes of 3 HCL-derived cell lines, Hair-M, JOK-1 and ESKOL, by merging a series of molecular cytogenetic methods, including conventional FISH, comparative genomic hybridization (CGH), and multiplex (multicolour) fluorescence ...