Evaluation of Appearance of Food Dyestuffs in a Variety of Solvents Present In Pharmaceutical Products
Evaluation of Appearance of Food Dyestuffs in a Variety of Solvents Present In Pharmaceutical Products
Abbreviations:
ADI - Acceptable Daily Intake
BP - British Pharmacopoeia
FAC - Food Advisory committee
UV-VIS - Ultra Violet - Visible
IARC - International Agency for Research on Cancer
TLC - Thin Layer Chromatography
HPLC - High Performance Liquid Chromatography
CE - Capillary Electrophoresis
Table of Contents
Abbreviations:2
Introduction4
Natural Food Dyes5
Synthetic Food Dyes5
Lake dyes5
Fast Green FCF:10
Quinoline Yellow:11
Sunset Yellow FCF:12
Chemistry of Colour14
Choice of Techniques:15
Instrumentation16
Project Aims:17
Reagents and Solutions18
Solvents used for analysis:18
Distilled Water18
Results and Discussion:19
Tartrazine in Distilled Water20
Tartrazine in IMS:23
Tartrazine in Chloroform Water (BP):25
Tartrazine in Chloroform Spirit (BP):27
Tartrazine is insoluble Chloroform30
Quinoline Yellow in Chloroform:33
Erythrosine B33
Fast Green FCF41
Quinoline Yellow48
Quinoline Yellow in Chloroform:52
Sunset Yellow FCF54
Influence of solvent on ?max (nm) of dyes61
Appearance of Blends:63
Fast Green FCF +Erythrosine B63
Fast Green FCF + Sunset yellow FCF:64
Conclusion:65
References65
Introduction
Over many years colorants have been used in the food, pharmaceutical and cosmetics industries. Adding colour to products makes them more eye-catching and easier to identify. Dyes are declared, water dissolvable synthetic nourishment colorants. They are constructed as powders, granules and liquids. The synthetic dyestuffs are of some kinds as per their rudimentary aggregate and structure.
The Anionic dyestuffs have a sulphonic or carboxilic assembly. The Catonic dyestuffs have amino or aminomethyl as the rudimentary assembly and are accessible as unpleasant salts. Azo dyestuffs have 1 to 3 azo bonds and make the biggest allowed nourishment dye. They have a prime aromatic amine and an aromatic aggregate and their blends cover a broad variety of colours.
These dyestuffs soak up lightweight and contemplate certain wavelength that is seen as its colour. Dyes have to be declared for their security to be utilised as a nourishment or cosmetic colorant. A dyestuff which is recorded as "FD&C" is allowed by the Food & Drug Administration to be supplemented to nourishment, pharmaceuticals and cosmetics. "D&C" entails the dyestuff may be utilised only in pharmaceuticals and cosmetics.
There are three major types of food colouring:
Natural Food Dyes
Natural Food Dyes are sourced from natural ingredients such as fruit, vegetables and minerals. Natural dyes have minimal harmful side effects; however they can be harmful to some who have allergies or intolerances to certain foods. Some examples of natural food colours are beetroot, carrot and turmeric.
Synthetic Food Dyes
Also known as artificial food colours; these are manufactured chemically in order to enhance the natural food colour associated with Pharmaceutical food products. Synthetic food colours such as tartrazine are argued to be harmful to humans due to its nitrous derivatives. Examples of where synthetic food dyes can be found include ice creams, fizzy drinks, cakes and pharmaceutical tablets.
Lake dyes
Lake dyes are similar to metal complexes; except in their solubility. Lake dyes are dispersible in oil whereas some synthetic dyes are not. They are fugitive, and so are replaced by other natural or synthetic colouring agents and therefore are useful in products such as cakes / biscuits which are made with fats / ...