DNA PROFILING AND ANALYSIS

DNA Profiling and Analysis

DNA Profiling and Analysis

Generally, the lower bounds of sensitivity suggested by manufacturers of short tandem replicate (STR) multiplex schemes are in the region of 250 pg. regardless, forensic scientists habitually request to boost the sensitivity of their procedures and the easiest way to do this is simply to lift the number of polymerase string of links reaction (PCR) amplification cycles. Findlay et al (1997) demonstrated that single (buccal) cells could be analyzed when 34 cycles were used with second generation multiplex (SGM) system. Interpretation was not clear-cut - added alleles were discerned, the dimensions of ...