[Conclusion]

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Conclusion

Conclusion

The present study clearly advances our knowledge of the inhibition of CCO with employers and their components, demonstrating for the first time that two components of the essential oil trepinen-4-ol and geraniol, may exert inhibitory action on the CCO membrane.

It may concluded as the study offers two new, specific anti-CCO compounds. It has been suggested that the effect of terpinen-4-ol and geraniol could be mediated by interaction with the plasma membrane, probably by inducing a reorganization of the architecture of lipids and loss of ions. The compounds are active against stationary phase cells that normally are not affected by antibiotics. Terpinne-4-ol and geraniol exhibited very rapid bactericidal activity in the CCO in the logarithmic phase. Leakage of cellular components parallel to the ion output indicating the action of these compounds is through the analysis and non-lethal. The activity of terpinen-4-ol and geraniol could be improved by the addition of a disruptor permeabilised membrane proton motive force. The reason for choosing this method as a typing method is based on a review of the literature in the introduction to this chapter. The protocol developed by Sullivan et al. (2006) was adopted for DNA extraction and automated system performance and conditions of the sequence. The PCR amplification conditions and primers used were selected based on information available at the MLST web site coordination (http://pubmlst.org/bcc).

Employers' organizations are complex mixtures and can produce multiple overlapping peaks when a traditional GC with a flame ionization detector is used, resulting in uncertainty of purity and quantification error, while mass spectrometry has the ability to recognize the peaks overlap and provide more reliable relative amounts of compounds. It can identify trace elements in materials that were previously beyond identification. Literature also reports (Lis-Balchin et al., 1997), incorporating the results from GC / MS, highlighting the importance of this method in the analysis of equal opportunities.

The principle of this method is that when a disk of filter paper impregnated with a test substance is placed on the agar, the test substance diffuses into the agar, producing a concentration gradient. The agency placed in the agar will not grow in the area around the disc if it is susceptible to the chemical. This area of no growth around the disk is known as the zone of inhibition. One of the major drawbacks of this method is that the volatile compounds of employers' organizations tend to evaporate and poorly soluble components can not diffuse well in agar. disk diffusion methods, however, can be recommended as a method of preliminary screening for antibacterial activity prior to more detailed studies because they are easy to make and only require a small amount of oil (Smith-Palmer et al. , 1998, Roengsumran et al., 1997). The size of the area provides an indication of the relative activity of the substance. However, many factors, such as media type, the amount of organism used, the concentration of the chemical and temperature of incubation of the influence of disk diffusion test.

This chapter describes the characterization methods used to ...