CAPILLARY ELECTROPHORESIS

Capillary Electrophoresis

Capillary Electrophoresis

Introduction

Electrophoresis refers to the movement (migration / transport) of charged particles in a usually liquid medium under the influence of an electric field. The migration rates of various ions depend on their charge, shape and size as well as of the effective solution environment and the strength of the electric field. Therefore, it comes as part of an electrophoretic separation of different ions. This can be used analytically and synthetically. This capillary separation takes place in a thin capillary tube held in an electrolyte solution (Whitesides, G. M, 2006, pp. 368-373).

Evaluation of Capillary Electrophoresis

Capillary electrophoresis (CE) may replace clinical laboratory methods such as electrophoresis, Southern blotting, sequencing and HPLC (High-performance liquid chromatography). It is an ideal analytical technique due to speed, possibility of handling great amount of samples, STI capacity to separate according to their small size molecules, charge, hydrophobic and stereo-specificity. The diagnosis of hereditary diseases or predisposition to polygenic diseases is related to polymorphisms mutations can be carried out with this method. In clinical laboratories, this technique is being used for the analysis of substrates present in urine. It is also a tool in forensic medicine firsthand for human identification and anthropology.

Capillary electrophoresis (CE) is a tool for separation of biomolecules, which in recent years has been very important in medicine. It has the versatility to separate amino acids, organic acids, inorganic ions, carbohydrates, steroids, thiols, food contaminants, material genetic and some important drugs in the study of different branches in the area of health (molecular diagnostics and clinical laboratory). It helps in the analysis of different analytes that can be performed in minutes, requiring just small amounts of sample in the range of nanoliters, with high reproducibility. It has a standard error of migration time of less than 0.5% (Lelievre F, Yan C and Zare RN, 1996, pp. 145-156).

Capillary Electrophoresis and DNA

Computer forensics involves the preservation, identification, extraction, documentation and interpretation of computer data Forensic sciences were practiced before they were identified as forensic anthropology or even forensic science. Forensic science was first documented in France in 1910 with Dr. Edmond Locard's establishment of a center for scientists studying biology, physics, and medicine came together to examine evidence for criminal investigations. This group analyzed materials and shared resources in an attempt to reconstruct crime scenes.

The scientific study of DNA evidence is not only important in crime films, but also in forensic every day. The introduction of DNA typing in the mid 1980-ies represented a quantum leap in forensics. The developed molecular biological techniques have now surpassed those places value of the traditional fingerprint at the end of the 19th Century. The police now hold an instrument in the hand that accelerates the identification and transfer of offenders especially in difficult cases where there is a lack of suspects, provides education only. The DNA analysis is also used in the same way, to the exclusion of suspects. For example, the FBI used every third investigation to elucidate the innocence of alleged suspects (Kuhn R and ...