BCA PROTEIN ASSAY

BCA Protein Assay

BCA Protein Assay

Introduction

The Bicinchoninic acid (BCA) method employs the reduction of Cu+2 to Cu+1 by protein in an alkaline medium. The combination of BCA and Cu+1 creates a purple-colored product that absorbs at 562 nm. The amount of product formed is dependent upon the amount of protein in the sample.

Individual proteins differ in their color responses, and one must consider this factor during protein analysis. Temperature, detergents, salts and various buffer components may affect the assay. The minimum concentration of Bovine Serum Albumin (BSA) read by the Modulus Absorbance Module and the BCA Protein Assay is ...